SPY650-FastAct_X
SPY650-FastAct_X (Also known as SiR-XActin1) is our most advanced live cell actin imaging probe. It labels highly dynamic actin filaments as well as the more static ones. SPY650-FastAct_X is a bright, fluorogenic, non toxic, cell permeable and highly specific live cell probe for fluorescence imaging of actin. SPY650-FastAct_X can label highly dynamic cellular actin structures but lacks undesired F-actin stabilization and toxic side effects. It does not require any transfection and works just by adding the probe to the cells and perform imaging a couple of hours later.
产品描述
SPY650-FastAct_X is a bright, far red, fluorogenic & non toxic F-actin probe based on our SPY™ dyes series. Its optimized actin ligand was engineered to avoid F-actin stabilization and fast exchange1). This unique feature renders SPY650-FastAct_X non-toxic and minimally perturbing cellular actin processes. It is able to label highy dynamic actin, just like LifeAct™** but the probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY650-FastAct_X is based on our bright, photostable & far red SPY650 fluorophore which is far superior than fluorescent proteins and highly STED comaptible.
SPY650-FastAct_X enables multicolor imaging with SPY505, SPY555, SPY595, SPY700, GFP or m-cherry. SPY650-FastAct_X can be imaged with standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging of living cells and tissue. Probe quantity allows 100 staining experiments.*
1) SiR-XActin: A fluorescent probe for imaging actin dynamics in live cells V. Nasufovic et al.; bioRxiv 2025.02.04.636537 *Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration. **LifeAct™ is a registered trademark of Ibidi GmbH.
产品参数
品名 SPY650-FastAct_X
货号 SC502
规格 100 stainings
λabs 652 nm
λfl 674 nm
lifetime 3.0 ns
推荐的STED损耗激光波长 775 nm
运输 常温
储存 -20℃
能否用于固定细胞 否
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